mouse monoclonal anti gas6  (R&D Systems)

Journal:

Article Title: GAS6/Mer axis regulates the homing and survival of the E2A/PBX1 positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche

doi: 10.1016/j.exphem.2009.11.002

Figure Lengend Snippet: (A) Expression of GAS6 receptor (Axl, Sky, Mer) mRNA in human leukemic cell lines as detected by real-time RT-PCR. (B) Mer protein levels expressed by human leukemic cells analyzed by flow cytometry. Cells were labeled with isotype-matched IgG control (solid line) or anti-Mer monoclonal antibodies (dotted line) before analysis. Data are presented the mean ± standard error of the mean and are representative of at least three independent experiments.

Article Snippet: The anti-human GAS6 antibody (goat IgG); the anti-human GAS6 monoclonal antibody (clone 100127, mouse IgG2a); and the anti-human Mer monoclonal antibody (clone 125518, mouse IgG1) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Expressing, Quantitative RT-PCR, Flow Cytometry, Labeling, Control

Journal:

Article Title: GAS6/Mer axis regulates the homing and survival of the E2A/PBX1 positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche

doi: 10.1016/j.exphem.2009.11.002

Figure Lengend Snippet: (A) Immunohistochemistry for GAS6 was performed on the primary human osteoblasts using monoclonal antibodies or isotype-matched IgG controls. (B) Quantitative digital image analyses of (A). Data are presented as the mean ± standard error of the mean. *Represents significant different from isotype-matched controls (P<0.05). (C) RCH-ACV cells (0-40000 cells) were seeded directly or indirectly onto 40000 primary human osteoblast monolayers in 24-well tissue culture plates in serum free condition. At 48 h, conditioned medium was collected and GAS6 protein levels were analyzed by ELISA. Data from a representative of three experiments are presented as the mean ± standard error of the mean. *Represents significant different from the primary human osteoblasts alone (P<0.05).

Article Snippet: The anti-human GAS6 antibody (goat IgG); the anti-human GAS6 monoclonal antibody (clone 100127, mouse IgG2a); and the anti-human Mer monoclonal antibody (clone 125518, mouse IgG1) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Immunohistochemistry, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: GAS6/Mer axis regulates the homing and survival of the E2A/PBX1 positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche

doi: 10.1016/j.exphem.2009.11.002

Figure Lengend Snippet: (A) Transwell™ migration assays were performed to determine if GAS6 (0-1 μg/ml) serves as a chemoattractant for RCH-ACV cells. Data are presented as mean ± standard error of the mean from triplicate determinations. BSA was served as negative control. *Represents significant different from spontaneous invasion (P<0.05). (B) Chemotaxis of RCH-ACV cells in response to GAS6 were analyzed in the presence or absence of antibodies that blocked the function of GAS6 or isotype-matched controls for 30 min at 4°C. RCH-ACV cells were also treated with either blocking anti-Mer antibody or isotype-matched control for 30 min at 4°C. After washing to remove excess antibodies, RCH-ACV cells were placed in the upper wells and allowed to migrate for 4 h. Data are presented as the mean ± standard error of the mean and as the percentage of migrated cells. *Represents significant difference from controls (spontaneous invasion or IgG control) (P<0.05).

Article Snippet: The anti-human GAS6 antibody (goat IgG); the anti-human GAS6 monoclonal antibody (clone 100127, mouse IgG2a); and the anti-human Mer monoclonal antibody (clone 125518, mouse IgG1) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Migration, Negative Control, Chemotaxis Assay, Blocking Assay, Control

Journal:

Article Title: GAS6/Mer axis regulates the homing and survival of the E2A/PBX1 positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche

doi: 10.1016/j.exphem.2009.11.002

Figure Lengend Snippet: (A) The effects of GAS6 on apoptosis of RCH-ACV cells were measured by annexin V staining. RCH-ACV cells were treated with GAS6 (1 μg/ml) or vehicle in the serum free media for 12 h. The percentage of apoptotic cells was assessed by flow cytometry. Data are presented as the mean ± standard error of the mean from three independent experiments. *Represents significant difference from vehicle treatments (P<0.05). (B) After 24 h incubation with GAS6 (1 μg/ml) or vehicle in 5% FBS condition, RCH-ACV cells were treated with AraC (0-10−3 M). At 48 h incubation, percentage of viable cells was assessed by XTT assay. Data are presented as the mean ± standard error of the mean from three independent experiments. *Represents significant difference from vehicle treatments (P<0.05).

Article Snippet: The anti-human GAS6 antibody (goat IgG); the anti-human GAS6 monoclonal antibody (clone 100127, mouse IgG2a); and the anti-human Mer monoclonal antibody (clone 125518, mouse IgG1) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Staining, Flow Cytometry, Incubation, XTT Assay

Journal:

Article Title: GAS6/Mer axis regulates the homing and survival of the E2A/PBX1 positive B-cell precursor acute lymphoblastic leukemia in the bone marrow niche

doi: 10.1016/j.exphem.2009.11.002

Figure Lengend Snippet: The effects of GAS6 on the cell-cycling state of RCH-ACV

Article Snippet: The anti-human GAS6 antibody (goat IgG); the anti-human GAS6 monoclonal antibody (clone 100127, mouse IgG2a); and the anti-human Mer monoclonal antibody (clone 125518, mouse IgG1) were purchased from R&D Systems (Minneapolis, MN).

Techniques: